Pluto Bioinformatics

PTPN2 knockout vs WT (GSE211636)

Bulk RNA-seq

We generated pluripotent stem cells (Mel1 hESC containing a GFP reporter driven by the endogenous insulin promoter) with a functional knock out of PTPN2 by CRISPR/Cas9 genome editing. KO or WT control stem cells were differentiated into beta-like cells (sBC), sorted for GFP, and prepared for deep sequencing. PTPN2 KO and WT sBCs were GFP+ sorted and prepared for bulk RNA sequence analysis.

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