Pluto Bioinformatics

GSE98884: Anti-inflammatory effect of indoleacrylic acid (IA)

Bulk RNA sequencing

Host factors in the intestine, such as mucus secretion, play an important role in selecting for the colonization of bacteria that contribute to intestinal health. Here we characterized the capability of commensal species to cleave and transport mucin-associated monosaccharides and found that several members of the Clostridiales order can utilize intestinal mucins as an energy source. One such mucin utilizer,Peptostreptococcus russellii,reduces susceptibility to epithelial injury in mice. Several Peptostreptococcusspecies contain a gene cluster that enables the production of the tryptophan metabolite indoleacrylic acid (IA), which we show has a beneficial effect on intestinal epithelial barrier function and mitigates inflammatory responses. Furthermore, metagenomic analysis of human stool samples revealed that the genetic capability of microbes to utilize mucins and metabolize tryptophan was diminished in patients with inflammatory bowel disease. Our data suggest that stimulating the production of IA to promote anti-inflammatory responses could have therapeutic benefit. SOURCE: Ramnik XavierXavier Lab Massachusetts General Hospital

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