Recent studies demonstrate that Ca2+ signaling has an important role in EMT. Use of Ca2+ blockers such as 2APB can inhibit cell migration induced by TGF-. Interestingly, we see an unexpected increase in Snail expression upon Ca2+ blocker treatment of both MCF10A and NMuMG cells; this increase is not observed with 2APB treatment alone. Therefore, we believe that 2APB plays a synergistic role with TGF- in Snail induction. We propose to investigate the gene networks that change following 2APB +TGF- treatment. SOURCE: Archana Dhasarathy (archana.dhasarathy@med.und.edu) -  University of North Dakota

Pluto Bioinformatics

GSE98596: NMuMG cell response to TGF-beta and TGF-beta +2APB treatments