Pluto Bioinformatics

GSE156496: Transcriptome analysis of the TA muscles from WT and Dmd Exon 51 Knockout mice

Bulk RNA sequencing

Duchenne muscular dystrophy (DMD) is a fatal muscle disorder characterized by cycles of degeneration and regeneration of multinucleated myofibers and pathological activation of a variety of other associated cell types. Here, we describe the creation of a new mouse model of DMD caused by deletion of exon 51 of the dystrophin gene, which represents a prevalent mutation in humans. To understand the transcriptional abnormalities and heterogeneity associated with the nuclei of myofibers, as well as other mononucleated cell types that contribute to DMD disease pathogenesis, we performed single nucleus transcriptomics of skeletal muscle of mice with exon 51 deletion. Our results reveal distinctive and previously unrecognized myonuclear subtypes within dystrophic myofibers and uncover degenerative and regenerative transcriptional pathways underlying DMD pathogenesis. Our findings provide new insights into the molecular underpinnings of DMD, controlled by the transcriptional activity of different types of muscle and nonmuscle nuclei. SOURCE: Zhaoning Wang (zhaoning.wang@utsouthwestern.edu) - Eric Olson Lab UT Southwestern

Dive into this experiment on Pluto.bio! Explore a myriad of analyses and visualizations, from differential expression and PCA to UMAP, t-SNE, gene set enrichment, and more. Discover insights through summary reports, coverage maps, clustering, and beyond. Also access to over 14,000 published experiments. Learn more