Pluto Bioinformatics

GSE95227: Gene expression profiling of ISWI chromatin remodeler mutants before and after DNA damage

Bulk RNA sequencing

Gene expression profiling of ISWI chromatin remodeler mutants before and after DNA damage BAZ1A is a regulatory subunit within the ISWI family of chromatin remodelers in humans. We and others have shown that BAZ1A is required for efficient recovery from DNA damage. The DNA damage hypersensitivity caused by BAZ1A* is as severe as the deletion of the entire gene. This is striking given that BAZ1A* contains only two substitutions (K1181A and K1183Y) within a poorly characterized domain of BAZ1A. We find that this domain can interact with DNA, and that the mutations present in BAZ1A* hinder DNA binding.; We used transcriptional profiling to identify molecular events leading to the DNA damage hypersensitivity of BAZ1A* cells. SOURCE: Sarah,K,Kummerfeld (kummerfeld.sarah@gene.com) - Genentech