The gut microbiome has garnered attention as an effective target to boost immunity and improve cancer immunotherapy. We found that B cell defective (BCD) mice, such as  membrane tail deletion (MT), have elevated anti-tumor immunity under specific-pathogen-free condition, depending on CD8+ T cells. Further studies suggest that microbiota-dependent upregulation of type-I IFN inducible genes, including Sca-1, in CD8+ T cells underlies the strong anti-tumor response of BCD mice.   Of the CD8+ T cell subpopulations, the most significant difference in Sca-1 expression between wild-type (WT) and BCD mice was seen in nave CD8+ T cells (CD44lowCD62Lhigh) in peripheral lymphoid and spleen. To further explore whether the upregulation of Sca-1 expression in peripheral CD8+ T cells of BCD mice associates with a unique gene profile, transcriptomic analysis was performed in peripheral CD8+ T cell populations in WT and MT mice at the single cell level, providing the evidence that among peripheral CD8+ T cells, nave CD8+ T cells are the most distinct subpopulation between WT and BCD mice. SOURCE: Kenji Chamoto Graduate School of Medicine, Kyoto University

Pluto Bioinformatics

GSE156338: Circulation of gut pre-activated nave CD8+ T cells enhances anti-tumor immunity in B cell defective mice