Pluto Bioinformatics

GSE156204: ext Generation Sequencing Facilitates Quantitative Analysis of Wild Type and MAP4K1-/- tumor inflitrating T cell Transcriptomes

Bulk RNA sequencing

Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare transcriptome profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRTPCR) methods and to evaluate protocols for optimal high-throughput data analysis; Methods: Tumor inflitrating T cell mRNA profiles of 21-day after inoculated in wild-type (WT) and Hematopoietic progenitor kinase 1 (HPK1/) mice were generated by deep sequencing, in duplicate, using Illumina HiSeq 2500. SOURCE: Bin Zou (zoubin@gzzoc.com) - State Key Laboratory of Ophthalmology Sun Yat-sen University

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