Pluto Bioinformatics

GSE93902: Effect of BCL11B knockdown on transcriptome of human T-cell precursors

Bulk RNA sequencing

To investigate the role of BCL11B in the initial stages of human thymopoiesis, we performed loss of function (knockdown) studies in an in vitro human thymopoiesis model (cord blood CD34+ cells co-cultured on OP9DLL1 stromal cell line). Gene expression profiling by RNA-Seq demonstrated that BCL11B knockdown resulted in downregulation of T-lineage genes and upregulation of stem cell, myeloid and NK genes, indicating BCL11B is required for the establishment of a T-lineage commitment transcriptional program. SOURCE: chintan parekh (cparekh@chla.usc.edu) - Children's Hospital Los Angeles