The cytokine IFN-g produced by tumor-reactive T cells is a key effector molecule with well characterized pleiotropic effects during anti-tumor immune responses. While IFN-g production is a transient event targeted at the immunological synapse, how IFN-g acts in time and space within the tumor microenvironment has remained elusive. Specifically, the link between duration of exposure to the cytokine and cellular response is poorly understood. Using RNA sequencing, we show that tumor cell phenotypic alterations require several hours of exposure to IFN-g, most likely due to a positive feedback loop mediated by STAT1 and IRF8. SOURCE: Philippe Bousso (philippe.bousso@pasteur.fr) -  Institut Pasteur

Pluto Bioinformatics

GSE140191: Bystander IFN- activity promotes widespread and sustained cytokine signaling altering the tumor microenvironment