Pluto Bioinformatics

GSE140066: Caco2 response to methotrexate, lactoferrin, and methotrexate-lactoferrin co-exposure

Bulk RNA sequencing

bulk sequencing outputs of Caco2 cells after exposure to permeability modifying and permeability rescuing agents.; To identify the molecular drivers for methotrexate-induced barrier dysfunction, we conducted RNA sequencing on Caco2 spheroids treated with methotrexate and lactoferrin. Given our observation that barrier function was compromised as early as 4-6 hours after exposure to methotrexate, we isolated RNA from spheroids 4-hour post-treatment to capture the transcriptional events responsible for initiating the processes. SOURCE: Thomas Wallach (thomas.wallach@gmail.com) - Gartner UCSF