In order to get the different gene expression profiles of these seminiferous tubules on different stages of spermatogenesis, four groups of samples were taken in accordance with the differentiation stage of germ cells, namely: empty tubes before SSCs transplantation (named as EmptBtrans), empty tubes after SSCs transplantation (named as EmptAtrans), tubes which SSCs had successfully homed and differentiated into spermatocytes and round spermatids (named as SM_and_RS) and fluorescent tubes which were taken from recipient mice that already had offsprings to make sure these tubes had complete spermatogenesis (named as sperm).  Pairwise comparison was made between these four groups, the number of significantly differentially expressed genes increased with the differentiation of germ cells. SOURCE: Xiaoyu Li (1103610135@qq.com) -  Institute of Zoology, Chinese Academy of Sciences

Pluto Bioinformatics

GSE93327: Transcriptome profiles of seminiferous tubes on different stage of spermatogenesis