Pluto Bioinformatics

GSE129366: Transnuclear mice reveal a population of Peyers patch iNKT cells that regulate B cell class switching to IgG1

Bulk RNA sequencing

Tissue resident iNKT cells maintain tissue homeostasis and peripheral surveillance against pathogens; however, studying these cells is challenging due to low abundance and poor recovery from tissues. To increase the number of tissue resident iNKT cells, we used transnuclear mice cloned from iNKT nuclei. We detected iNKT cells from tissues where they are rare, including adipose, lung, skin-draining lymph nodes, and a previously undescribed population in Peyers patches (PP). We characterized the transcriptional profile of this novel population by bulk RNA-Seq. SOURCE: Stephanie Dougan (stephanie_dougan@dfci.harvard.edu) - Dana-Farber Cancer Institute