Pluto Bioinformatics

GSE129106: Intraductal Adaptation of the 4T1 Mouse Model of Breast Cancer Reveals Effects of the Epithelial Microenvironment on Tumor Progression and Metastasis

Bulk RNA sequencing

Tumors were harvested at day 24 post tumor cell engraftment and dissociated into single-cell suspensions. RFP-positive 4T1 cells were isolated by fluorescence-activated cell sorting (FACS). RNA was extracted using the RNeasy Mini Kit. Three independent RNA preparations from cultured 4T1-Luc2-RFP cells and FACS-isolated 4T1-Luc2-RFP cells were submitted for RNA sequencing analysis. SOURCE: Ann Ramsdell (ann.ramsdell@uscmed.sc.edu) - University of South Carolina