Pluto Bioinformatics

GSE126780: RNA sequencing of isogenic BRCA2 haploinsufficient vs. wild-type T-ALL cells

Bulk RNA sequencing

We found a high frequency of heterozygous Fanconi-BRCA pathway mutations in pediatric T-ALL. BRCA2 was the most commonly mutated gene. We transduced Cas9-expressing Jurkat cells, which lacked an identifiable BRCA2 mutation, with an integration-defective lentiviral guide RNA expression construct targeting exon 11 of BRCA2 (NM_000059). Single-cell cloning and sequencing analysis revealed two distinct clones harboring monoallelic BRCA2 frameshift mutations, termed clones W4 and W5. Each of these clones was subjected to RNA sequencing analysis. SOURCE: Alejandro Gutierrez Boston Children's Hospital