To determine whether histone deacetylase (HDAC) inhibition has genome-wide effects on gene expression, we used RNA sequencing to analyze the mRNA profile in prefrontal cortex (PFC) of wild-type (WT) and Shank3-deficient (Shank3+/C) mice treated with romidespin or saline control. We mapped the sequences to 24,420 mouse genes. Compared to WT, 365 genes in saline-treated Shank3+/C mice showed a change in expression values of at least 1.2 fold and p  0.01 (213 downregulated, 152 upregulated). In romidepsin-treated Shank3+/C mice, ~88% of the downregulated genes (n=187 genes) were normalized to control values. Further analysis of the romidepsin-restored genes revealed enrichment in actin cytoskeleton-mediated transport, signal transduction pathways, and developmental processes. SOURCE: Zhen Yan (zhenyan@buffalo.edu) -  University at Buffalo

Pluto Bioinformatics

GSE109328: Epigenetic Rescue of Autism-like Social Deficits in Shank3-Deficient Mouse Models