Pluto Bioinformatics

GSE149251: Next Generation Sequencing Facilitates Quantitative Analysis of MV4-11 cell line Transcriptomes

Bulk RNA sequencing

Purpose: The goals of this study are to compare side population and main population cells transcriptome profiling (RNA-seq) .; Methods: We sorted side population and main population cells by staining Hoechst 33342 and using Flow Cytometer (Beckman Coulter, Moflo XDP). 200,000 cells per sample were analysed in RNA-Seq experiments.The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: BurrowsWheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRTPCR validation was performed using TaqMan and SYBR Green assays SOURCE: Fangfang Wang ( - West China Hospital, Sichuan University

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