Pluto Bioinformatics

GSE97471 (human): Differentially expressed genes from RNA-Seq and functional enrichment results are affected by the choice of single-end versus paired-end reads and stranded versus non-stranded protocols

Bulk RNA sequencing

We undertook four mammalian transcriptomics experiments to compare the effect of read mapping, feature counting and differential expression analysis using single-end (SE) and paired-end (PE) protocols. For three of these experiments we also compared a non-stranded (NS) and a strand-specific approach to mapping the paired-end data. SOURCE: Susan Corley (s.corley@unsw.edu.au) - Systems Biology Initiative UNSW