PHF8 exerts distinct functions in different types of cancer. However, the mechanisms underlying its specific functions in each case remain obscure. To establish whether overexpression of PHF8 regulates the TGF- induced the epithelial-mesenchymal transition (EMT), we treated MCF10A-Mock (control) and MCF10A-PHF8wt (overexpressing wild-type PHF8) cells with TGF-1 for 0, 24, 48 and 72 hours and performed RNA-seq in biological duplicates. Our data indicated that EMT gene signatures were significantly enriched in MCF10A-PHF8 cells with TGF-1 treatment at all time points, strongly indicating that PHF8 overexpression induces a sustained EMT signaling program. SOURCE: Hank Qi (hank-qi@uiowa.edu) - Qi lab The university of Iowa

Pluto Bioinformatics

GSE74377: Overexpression of PHF8 promotes an EMT-related gene signature in MCF10A cells