Pluto Bioinformatics

GSE141745: Tissue neutrophil heterogeneity

Bulk RNA sequencing

Purpose: The goals of this study are to compare bulk RNAseq profiles of tissue neutrophils.; Methods: Bulk RNAseq of sorted neutrophils from bone marrow, spleen, blood, lung, peripheral blood, skin and intestine from wild-type (WT) mice, in triplicate, using Illumina. The sequence reads that passed quality filters were analyzed at the gene level with RSEM which mapped about 26 million sequence reads per sample to the mouse genome (build mm10/GRCm38) and identified 32328 genes. SOURCE: Andrés HidalgoImaging of the Cardiovascular Inflammation and the Immune Response Spanish National Center for Cardiovascular Research