Pluto Bioinformatics

GSE105781: RNA-seq expression profiling of Grhl2-deficient and control murine lung epithelium at embryonic day E16.5

Bulk RNA sequencing

Epithelial (CD31-CD45-EpCAM+) cells were isolated by FACS from Grhl2-deficient (Shh-Cre;Grhl2f/-) and control (Shh-Cre;Grhl2f/+) embryonic lungs at day E16.5 (3 biological replicates/genotype). Total RNA extracted from the samples was subjected to next-generation sequencing (NGS) library preparation using standard Illumina protocols. Completed libraries from individual samples were sequenced on a HiSeq2500 at the Australian Genome Research Facility. SOURCE: Matthew Ritchie (mritchie@wehi.edu.au) - The Walter and Eliza Hall Institute of Medical Research