Obtaining highly purified differentiated cells via directed differentiation from human pluripotent stem cells (hPSCs) is an essential step for the clinical application of hPSCs. Among the various conditions that should be optimized, the precise role and contribution of the extracellular matrix (ECM) during differentiation are relatively unclear. Here, using a short fragment of laminin 411 (LM411-E8), an ECM predominantly expressed in the vascular endothelial basement membrane, we demonstrated that the directed switching of defined ECMs robustly yields highly purified (>95%) endothelial progenitor cells (PSC-EPCs) from hPSCs in integrin-laminin axis and rho signaling pathway-dependent manner. SOURCE: Akira Watanabe (a.watanabe@cira.kyoto-u.ac.jp) - Department of Life Science Frontiers Kyoto University

Pluto Bioinformatics

GSE85782: Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells [Bulk RNA-Seq]