In mammals body temperature fluctuates diurnally around a mean value of 36-37C. Despite the small differences between minimal and maximal values, body temperature rhythms can drive robust cycles in gene expression in cultured cells and, likely, in, animals. Here we studied the mechanisms responsible for the temperature-dependent expression of Cold- Inducible RNA-Binding Protein (CIRBP). In NIH3T3 fibroblasts exposed to simulated mouse body temperature cycles Cirbp mRNA oscillates about 3-fold in abundance, as it does in mouse liver. This daily mRNA accumulation cycle is directly controlled by temperature oscillations and does not depend on the cells circadian clocks. Here, we show that the temperature-dependent accumulation of Cirbp mRNA is controlled primarily by the regulation of splicing efficiency, defined as the fraction of Cirbp pre-mRNA processed into mature mRNA. As revealed by genome-wide approach-to-steady-kinetics, this posttranscriptional mechanism is wide-spread in the temperature-dependent control of gene expression. SOURCE: Felix Naef (felix.naef@epfl.ch) - Felix Naef École Polytechnique Fédérale de Lausanne

Pluto Bioinformatics

GSE85553: Temperature regulates splicing efficiency of the cold-inducible RNA-binding protein gene Cirbp