Pluto Bioinformatics

GSE104790: Hemogenic endothelial fate mapping reveals dual developmental origin of mast cells.

Bulk RNA sequencing

Hematopoiesis occurs in distinct waves. Definitive hematopoietic stem cells (HSC) with the potential for all blood lineages emerge in the aorta-gonado-mesonephros (AGM), while primitive progenitors, whose potential is thought to be limited to erythrocytes, megakaryocytes and macrophages (M), arise earlier in the yolk sac (YS). Here, we questioned whether other YS lineages exist that have not been identified, partially owing to limitations of current lineage tracing models. We established the use of Cdh5CreERT2 for hematopoietic fate mapping, which revealed the YS origin of mast cells (MC). YS derived MC are replaced by definitive MC, which maintain themselves independently from the BM in the adult. Replacement occurs with tissue specific kinetics. MC in the skin, but not other organs, remain largely YS derived prenatally and are phenotypically and transcriptomically distinct from definite adult MC. We conclude that dual hematopoietic origin is not M specific, but shared between these two myeloid lineages. SOURCE: jinmiao chen (chen_jinmiao@immunol.a-star.edu.sg) - Singapore Immunology Network