Pluto Biosciences, Inc

GSE153258: Global RNA stability profiling during selective chemical inhibition of human Dcp2

Bulk RNA sequencing

Multiple RNA decapping enzymes coexist in mammalian cells to regulate decay of partially overlapping sets of cellular transcripts, but a comprehensive understanding of cellular substrate selectivity of each enzyme is yet to be achieved. Previously we demonstrate the utility of TimeLapse-seq in global profiling of RNA stability changes in human Dcp2 knockout cells. However, secondary transcriptional changes and upregulation of alternative decay pathways have obscured complete mapping of Dcp2 substrates. Here, we present the discovery and first application of a cell-permeable, highly selective Dcp2 ligand in the chemical genetic study of its RNA substrates. SOURCE: Sarah Slavoff (sarah.slavoff@yale.edu) - Yale University

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