Pluto Bioinformatics

GSE83512: Transcriptome-wide analysis to determine miR-200a targets in melanoma cell lines

Bulk RNA sequencing

Three metastatic melanoma cell lines (BD-0548-ME, DP-0574-ME and WP-0614-ME) were transfected with miR precursors; we used hsa-miR negative control (NC) or hsa-miR-200a-3p precursors (miR-200a). The objective of this experiment was to determine miR-200a tagerts in melanoma cell lines. We selected low -miR-200a expression cells and then we overexpresed miR-200a or NC. Finally, we compared metastatic melanoma cells overexpressing miR-200a (miR-200a) to the same cell line but overexpressing negative control miR (NC). mRNA profiles were generated by deep sequencing using Illumina HiSeq 2500. SOURCE: Dave Hoon John Wayne Cancer Institute

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