Pluto Bioinformatics

GSE136376: scRNA-seq of LPS responding WT or IRF4-deficient B cells

Bulk RNA sequencing

To fine map the continuum of molecular changes that occur as B cells differentiate to antibody secreting plasma cells in response to LPS we performed scRNA-seq. WT or IRF4-deficient B cells were CTV labeled and adoptively transferred into congenically disparate uMT hosts. One day later, the mice were innoculated with LPS and all transferred cells isolated at 72 hours and profiled on the 10x Genomimcs 5' Library Kit. SOURCE: Chris Scharer (cdschar@emory.edu) - Chris Scharer Emory University