Pluto Bioinformatics

GSE98133: Determination of Lsd1 function in C2C12 myoblast cells by global transcriptome analysis

Bulk RNA sequencing

First, we aimed to identify genes whose transcript levels change at the onset of myogenic versus adipogenic differentiation of muscle precursors. We therefore compared RNA-seq results obtained from C2C12 cells differentiated for 1 day in myogenic and adipogenic medium. Out of these genes, we wanted to determine those whose expression was affected by altered Lsd1 levels. To this end, we performed RNA-seq in C2C12 cells upon LSD1 overexpression and Lsd1 knock-down in adipogenic medium and compared them to corresponding control cells. SOURCE: Roland Schuele (roland.schuele@uniklinik-freiburg.de) - Schuele Zentrale Klinische Forschung