Pluto Bioinformatics

GSE120229: m6A-seq data analysis of control and PCIF1 knockdown transcriptome

Bulk RNA sequencing

Purpose: To identify the in vivo RNA targets of PCIF1, we performed meRIP experiments for PCIF1 KD and control cells using an anti-m6A antibody. We envisioned that the cap-specific PCIF1 should selectively alter the m6Am modification at the 5-terminal region of transcripts. Indeed, we observed a reduction of modification peaks at the 5-end but not the 3-UTR regions of mRNA upon PCIF1 KD. SOURCE: Kai Li (li_kai@pku.edu.cn) - Yi Lab Peking University