Pluto Bioinformatics

GSE70499: Effect of Bmal1 pre-natal knockout on liver gene expression: time-series

Bulk RNA sequencing

We use a conventional (pre-natal) Bmal1 KO (cKO) mouse strain to investigate the effect of circadian disruption on gene expression in the liver by comparing cKO mice with wild type (WT) mice using RNA-Seq. All mice were housed under 12h:12h LD conditions with free access to food and water. Three mice per genotype were sacrificed every 4 h for 20 h (6 time points). Illumina TruSeq Stranded mRNA Sample Prep Kit Set A was used for library construction and samples were sequenced on an Illumina HiSeq 2500. RNA-seq data were aligned by STAR, and data were normalized with a resampling strategy (https://github.com/itmat/Normalization). SOURCE: Georgios,K,Paschos (gpaschos@mail.med.upenn.edu) - FitzGerald University of Pennsylvania, School of Medicine