Pluto Bioinformatics

GSE100082: Smad4 pathways modulate induction of the chemokine Ccl20 and repress inflammation-induced carcinogenesis in mouse colon

Bulk RNA sequencing

To understand the extent of Smad-mediated gene regulation in the colon, we isolated colon epithelium from Smad4Lrig1 and from Smad4+ control mice (either mice lacking a CreERT allele and treated with tamoxifen, or mice bearing a CreERT allele but treated with vehicle only) and analyzed the colonic epithelium by RNAseq. The ability of TGF1 and/or BMP2 to block TNF-mediated induction of Ccl20 from our study suggests that these Smad-mediated pathways may act as gatekeepers for induction of other inflammation-associated genes. To determine if Smad-mediated signaling blocks all or specific subsets of TNF-induced genes, we analyzed both colonocytes and mouse colonoid treated with or without TNF, TGF1, and BMP2 by RNA seq. SOURCE: Jing Zhu (jing.zhu@vanderbilt.edu) - Vanderbilt University