Pluto Bioinformatics

GSE136340: Effects of darunavir upon gene expression in kidney tubular cells after transduction with HIV or EGFP-control lentivirus

Bulk RNA sequencing

To determine the effect of darunavir upon HIV-induced gene regulation in renal epithelial cells, immortalized human renal tubular epithelial cells (HPT1b) were transduced with VSV-pseudotyped gag/pol-deleted HIV lentivirus or EGFP control lentivirus. 4 days later, cells were treated with darunavir (3uM) or vehicle control from 4-7 days post transduction. Primary comparisons in gene expression were between HIV and control-transduced cells at 4 days post transduction, and between DRV and control-treated HIV-transduced cells at 7 days post transduction. SOURCE: Weijia Zhang (weijia.zhang@mssm.edu) - Bioinfomatics Icahn School of Medicine at Mount Sinai