Pluto Bioinformatics

GSE110144: T cells activated in the presence or absence of TLR agonists

Bulk RNA sequencing

We report the gene expression differences between OT-1 T cells activated alone or in the presence of TLR1/2 (Pam3CSK4) or TLR7 (Gardiquimod). By activating OT-1 splenocytes in the presence or absence of the TLR agonists, isolating RNA from purified CD8+ T cells we show that cells activated in the presence of either TLR1/2 or TLR7 agonists had similar transcriptional profiles demonstrating an increase in Th1 cytokine expression over cells that were activated alone. This study provides a key piece of information for those designing T cell mediated therapies. SOURCE: Douglas,G.,McNeel (dm3@medicine.wisc.edu) - University of Wisconsin