TDP43 and SRSF3 has been reported to be RNA-binding proteins; however their roles in breast cancer progression has not been  examined previously. Here, we performed RNA-seq on MDA-MB231 cells stably expressed sh-control, shTDP43, shSRSF3 or sh-TDP43 and sh-SRSF3 using lentivirus in duplicates. In addition, MDA-MB231 cells with stable expression of flag-TDP43 or flag-SRSF3 were also generated by using lentivirus. RIP-seq was also applied to identify binding RNA against Flag antibodies. SOURCE: Baowei Jiao (jiaobaowei@mail.kiz.ac.cn) - State Key Laboratory of Genetic Resources and Evolution Chinese Academy of Sciences

Pluto Bioinformatics

GSE98016: Transcriptome analysis on TDP43 and SRSF3 downstream genes and binding RNAs in MDA-MB231 cells by Next Generation Sequencing