Pluto Bioinformatics

GSE147496: RNA-seq of primary mouse B cell progenitor populations after activation with LPS

Bulk RNA sequencing

Naive resting B-cells were purified from bone marrow from C57BL/6 mice using flow cytometry sorting. Naive B cells were activated with LPS and harvested 3 hours, 10 hours or 33 hours after the LPS treatment. Expanded activated B cells (CD22+ CD138-) and plasmablasts (CD22- CD138+) were also harvested on day 4 post-stimulation. SOURCE: Gordon,K,Smyth (smyth@wehi.edu.au) - Smyth Walter and Eliza Hall Institute of Medical Research