N6-Methyladenosine (m6A) and N6,2-O-dimethyladenosine (m6Am) are abundant mRNA modifications that regulate transcript processing and translation. The role of both, here termed m6A/m, in the human stress response are currently unknown.  Here, we provide m6A/m-Seq of immortalied cell lines derived from B lymphocytes from male healthy donors or male donors diagnosed with major depressive disorder (MDD), harvested 1 h after treatment with 100 nM cortisol or mock treatment. PolyA-RNA-fragments of each sample was processed both as m6A/m-sample (RNA immunoprecipiation RIP with an m6A and m6Am antibody) and RNA-input sample. SOURCE: Mareen Engel (mareen_engel@psych.mpg.de) -  Max-Planck-Institute of Psychiatry

Pluto Bioinformatics

GSE113798: m6A/m-Seq of human B-lymphocyte cell lines from healthy controls and major depressive disorder patients