Pluto Bioinformatics

GSE154786: RNA-sequencing analysis identifies BRLF1-dependent viral and cellular transcriptome during EBV primary infection in B lymphoma cells

Bulk RNA sequencing

Purpose: Immediate-early protein BRLF1 plays an important role in lytic infection of Epstein-Barr virus (EBV), it activates lytic viral transcription and viral DNA replication, affects cell cycle and suppresses inflammatory responses. However, the understanding of BRLF1 influence on cellular gene expression during early lytic cycle remains limited.; Methods:RNA-sequencing analysis identified the differentially expressed genes (DEGs) in B lymphoma cells undergoing wild type and BRLF1-deficient EBV primary infection. The libraries were sequenced on an Illumina HiSeq-Xten platform and 150 bp pairedend reads were generated. An average read depth of 12G reads per sample was collected and analyzed. Data analysis was performed with Molecule Annotation System 3.0.; Results: A total of 159 unigenes were annotated and the major differentially enriched pathways were related to DNA replication and transcription, immune and inflammatory response, cytokine-receptor interaction and chemokine signaling, ECM-receptor interaction and focal adhesion. Further the mass spectrometry identified the BRLF1-binding proteins and showed that BRLF1 employed different strategies to influence RNA polymerase II-dependent viral and cellular transcription, by binding to a particular RREs motif and binding to unique transcription factors which binding to specific motifs.; Conclusions: Our study characterized the BRLF1-dependent cellular and viral transcriptional profile during primary infection and then revealed the important virus-cell interaction and alterations of cell activity for EBV primary infection and lytic replication. SOURCE: Ersheng Kuang (kuangersh@mail.sysu.edu.cn) - Sun Yat-Sen University

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