Pluto Bioinformatics

GSE56267: The age and genomic integrity of neurons after cortical stroke in humans

Bulk RNA sequencing

It has been unclear whether ischemic stroke induces neurogenesis or neuronal DNA-rearrangements in the human neocortex. We show here that neither is the case, using immunohistochemistry, transcriptome-, genome- and ploidy-analyses, and determination of nuclear bomb test-derived 14C-concentration in neuronal DNA. A large proportion of cortical neurons display DNA-fragmentation and DNA-repair short time after stroke, whereas neurons at chronic stages after stroke show DNA-integrity, demonstrating the relevance of an intact genome for survival. SOURCE: Joakim Lundeberg KTH-Science for Life Laboratory