The piRNA biogenesis was evaluated in the mouse testes lacking TEX15. Total small RNAs, MILI-, and MIWI2-associated small RNAs from control (Tex15+/-) and Tex15(-/-) knock-out testes were sequenced. Data analysis revealed that the abundance of miRNAs and piRNAs was comparable between control and Tex15 knock-out testes, suggesting that loss of TEX15 function does not impair piRNA biogenesis. However, the relative abundance of MILI- and MIWI2-bound sense piRNAs derived from transposable elements was increased. SOURCE: Ramesh Pillai (ramesh.pillai@unige.ch) -  University of Geneva

Pluto Bioinformatics

GSE107832: Epigenetic control of transposable elements in the mouse male germ line by Tex15