Pluto Bioinformatics

GSE137414: RNA sequencing of pancres tissue of WT and IL23 expression mice (KSR23)

Bulk RNA sequencing

The goal of this study is to check if elevated level of IL-23 and/or its downstream cytokines affect the gene expression in the pancreas of newborn mice. We engineered a mice strain in which the expression of IL-23 was directed to the keratinocytes (KSR23). The KSR23 mice had normal body weight at birth and much smaller than their control littermates at day 5. KSR23 mice died prematurely (before 15 days of age), but displayed no signs of disease in the skin, intestine, or in other organs examined. To investigate if elevated level of IL-23 and/or its downstreamcytokines could affect body growth, we examined the transcriptome of the the pancreas, which are critical for the processing and absorption of nutrients. Pancreatic mRNA profiles of 5-day-old WT and KSR23 were generated by deep sequencing. The transcriptome of the pancreas of KSR23 mice at P5 differed significantly from that of their control littermates (WT). Kegg pathway analysis showed that several genes involved in pancreatic secretion, were downregulated in the pancreas of KSR23 mice. The pancreatic secretion pathway includes genes encoding several pancreatic enzymes involved in food digestion and genes involved in the secretory pathway. In conclusion, increased expression of IL-23 and/or its down cytokines affects expression of genes in neonatal pancreas. SOURCE: Sergio Lira (sergio.lira@mssm.edu) - Icahn School of Medicine at Mount Sinai