Pluto Bioinformatics

GSE113885: Multi-axial self-organisation properties of mouse embryonic stem cells into gastruloids [E6.5 to E9.5]

Bulk RNA sequencing

Gastruloids are small aggregates of murine Embryonic Stem (ES) cells stimulated to undergo gastrulation-like symmetry breaking events in vitro (van den Brink, S. C. et al. 2014, Simunovic, M. & Brivanlou, A. H 2017). We analyzed the transcriptomic profiles of gastruloids at different time-points after aggregation (AA) and compared them with those of mouse embryo at different stages, raging from e6 to e9.5. We show that gastruloids are capable of organizing a post-occipital pattern of neural, mesodermal and endodermal derivatives that mimics that of the embryo in terms of gene expression. SOURCE: Leonardo Beccari (leonardo.beccari@unige.ch) - University of Geneva