Pluto Bioinformatics

GSE111600: Exploiting Mouse Models to Understand Female Hypersensitivity to Cocaine

Bulk RNA sequencing

Male mice, female mice, ovariectomized female mice given placebo pellets, and ovariectomized female mice given extradiol pellets were treated with experimenter-administered saline or cocaine for 7 days, and behaviorally monitored. After a 28 day withdrawal period, RNA samples were prepared from nucleus accumbens and subjected to RNA-Seq, followed by differential expression analyses using Cuffdiff, Limma and DESeq2. Selected transcripts were further investigated using quantitative polymerase chain reaction. SOURCE: Richard,E,Mains (mains@uchc.edu) - University of Connecticut Health Center