Sp5 is a target and critical transducer of the Wnt/beta-catenin signaling pathway and is essential for mesoderm differentiation in vertebrate embryos. We established a doxycycline inducible ES cell line to over-express Flag epitope-tagged Sp5 protein in differentiating ES cells which have the capacity to form mesoderm cells in vitro. The goal of this study was to identify the genes/gene networks regulated by Sp5 to understand how it regulates mesoderm differentiation by identifying important target genes which may mediate its activity. SOURCE: Mark,W,Kennedy (mark.kennedy@nih.gov) - Cell Signaling in Vertebrate Development NIH/NCI

Pluto Bioinformatics

GSE73082: Determination of the Sp5 regulated transcriptome in differentiating ES cells