Background:  Tuberculosis (TB) remains a major public health problem, especially in developing countries, with 1.5 million deaths annually worldwide. Macrophages (Ms) are central to TB pathogenesis. Ms play a key role in the outcome of the infection. More importantly, Ms are the primary cell target of MTB, which has developed different strategies to multiply inside the M phagosome. Here we aim to compare the transcriptome of Mycobacterium tuberculosis (MTB)-infected Ms with that of cells stimulated with heat-killed MTB.;	Results: Here, we used live and heat-inactivated MTB to examine the impact of infection on the human macrophage response. Gene expression profiling has revealed about 3,259 genes differentially expressed in macrophages incubated with heat-inactivated MTB and 4,179  genes differentially expressed with live MTB. We found that the response to live and heat-inactivated MTB was strongly correlated (r>0.78).;	Conclusions: Our results show that macrophages respond in a similar manner to live and heat-inactivated MTB.  We also identified some genes differentially expressed only in one condition. SOURCE: Alexandre Giraud-Gatineau (alexandre.giraud.gatineau@gmail.com) - integrated mycobacterial pathogenomics Institut Pasteur

Pluto Bioinformatics

GSE143627: Inactivated M. tuberculosis and M.tuberculosis Infection remodels the macrophage response