Pluto Bioinformatics

GSE63424: Sequencing of First-strand cDNA Library Reveals Full-length Transcriptomes

Bulk RNA sequencing

We developed a new method of preparing libraries for strand-specific RNA sequencing (ssRNA-Seq). It employs Direct Ligation of Adaptors to First-strand cDNA (DLAF). We compared ssRNA-Seq libraries prepared using either the DLAF and dUTP methods from mouse embryonic stem cells (mES) and libraries were sequenced from one end or both ends. We also conducted a comparison of ssRNA-Seq libraries prepared using DLAF and ScriptSeq v2 kit (Epicenter) from mouse embryonic cortex (mECx). SOURCE: Saurabh Agarwal (saurabha@umich.edu) - Shigeki Iwase University of Michigan