Pluto Bioinformatics

GSE89503: PC1/3 deficiency impacts POMC processing in human embryonic stem cell-derived hypothalamic neurons

Bulk RNA sequencing

We developed a technique for generating hypothalamic neurons from human pluripotent stem cells. Here, as proof-of-principle, we examine the use of these cells in modeling of a monogenic form of severe obesity: PCSK1 deficiency. We generated PCSK1 (PC1/3)-deficient human embryonic stem cell (hESC) lines using both shRNA and CRISPR-Cas9, and investigated pro-opiomelanocortin (POMC) processing using hESC-differentiated hypothalamic neurons. SOURCE: Yurong Xin (yurong.xin@regeneron.com) - Regeneron Pharmaceuticals, Inc.