Pluto Bioinformatics

GSE75297: DIGIT regulates endoderm differentiation of human embryonic stem cells

Bulk RNA sequencing

Endoderm differentiation is a critical step leading to development of the gastrointestinal system. We found that DIGIT is a lncRNA that is required for endoderm differentiation. We used directional RNA sequencing (RNA-seq) to globally evaluate the effects of loss of DIGIT expression during endoderm differentiation and chromatin immunoprecipitation and sequencing (ChIP-seq) to define the sites occupied by SMAD3 during endoderm diferentiation. SOURCE: Chan Zhou (zhou.chan@mgh.harvard.edu) - Alan Mullen Mass General Hospital, Harvard Medical School