Pluto Bioinformatics

GSE89479: Self-organized amniogenesis by human pluripotent stem cells in a biomimetic implantation-like niche

Bulk RNA sequencing

In this work, we generated early human amnion-like tissues by culturing human pluripotent stem cells (hPSCs) in a bioengineered implantation-like niche in vitro. To explore the gene expression profile of hPSC-derived amnion-like cells (hPSC-amnion), we performed mRNA-sequencing for both undifferentiated hPSCs and hPSC-amnion. Here we show that hPSC-amnion differs from hPSCs by actively regulating a comprehensive set of transcriptional regulation network and developmental signaling pathways such as BMP-SMAD signaling. SOURCE: Katherine Gurdziel (gurdziel@wayne.edu) - Wayne State University