In this study we aimed to assess technical variability associated with globin depletion in addition to assessing general technical variability in RNA-Seq from whole blood derived samples. We compared technical and biological replicates having undergone globin depletion or not and found that globin depletion removed approximately 80% of globin transcripts, improved the correlation of technical replicates, allowed for reliable detection of thousands of additional transcripts and generally increased transcript abundance measures. SOURCE: Scott Tebbutt (scott.tebbutt@hli.ubc.ca) -  PROOF Centre of Excellence

Pluto Bioinformatics

GSE53655: Variation in RNA-Seq transcriptome profiles of peripheral whole blood from healthy individuals with and without globin depletion