Pluto Bioinformatics

GSE86418: Transcriptomic profiling of dorsal root ganglia of transgenic sickle and control mouse models for studying genetic signatures of pain in sickle cell disease

Bulk RNA sequencing

We sequenced mRNA from the dorsal root ganglion (DRG) of transgenic mouse models of sickle cell disease (SCD) and their transgenic controls. Each genotype (strains) were divided into two age groups and each age groups were subjected to two treatment conditions (each simulating acute and chronic pain respectively). Each of these 8 groups had 6 male mice (except one group had 7 mice) resulting into a total of 49 samples. The goal of mRNA sequencing of these samples is to identify genetic signatures associated with transition from acute to chronic pain in SCD. SOURCE: Kalpna Gupta University of Minnesota