Pluto Bioinformatics

GSE114166: TWEAK/Fn14 axis is a therapeutic target for post-angioplasty restenosis

Bulk RNA sequencing

Aim: Next generation sequencing-based methods were performed to identify genes and pathways regulated by TWEAK in VSMCs; Methods: Using a gene-set enrichment method, we found a functional module involved in cell proliferation defined as the minimal network connecting top TWEAK up-regulated genes.; Results: TWEAK increased the number of VSMCs in S phase and the total number of proliferative cells.; Conclusions: Our data define a major role of TWEAK/Fn14 in the control of VSMCs proliferation and migration during neointimal hyperplasia after wire injury in mice, and identify TWEAK/Fn14 as a potential target for treating restenosis after angioplasty. provide a framework for comparative investigations of expression profiles. Our results show that NGS offers a comprehensive and more accurate quantitative and qualitative evaluation of mRNA content within a cell or tissue. We conclude that RNA-seq based transcriptome characterization would expedite genetic network analyses and permit the dissection of complex biologic functions. SOURCE: Julio Madrigal-Matute ( - Albert Einstein College of Medicine

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